Business and Management

Why Should You Buy a Cell Counting Kit?

Summary

Cell counting kit-8 or CCK-8 allows easy assays utilizing WST-8, monosodium salt, that generates a water-soluble formazan dye upon bio reduction from the existence of an electron carrier, 1-Methoxy PMS. CCK-8 alternative is added directly into the cells, so no […]

Cell counting kit-8 or CCK-8 allows easy assays utilizing WST-8, monosodium salt, that generates a water-soluble formazan dye upon bio reduction from the existence of an electron carrier, 1-Methoxy PMS. CCK-8 alternative is added directly into the cells, so no pre-mixing of elements is necessary. 

WST-8 is bio reduced by mobile dehydrogenases to an orange formazan product that's soluble in tissue culture medium. The quantity of formazan produced is directly proportional to the amount of cells. Cell Counting equipment enables sensitive colorimetric assays for the determination of the amount of possible cells from the proliferation and cytotoxicity assays. 

The detection sensitivity is significantly greater than every other tetrazolium salts like MTT, XTT. WST-8, is decreased from dehydrogenase activities in cells to provide a yellow-color formazan dye, which can be soluble in the tissue culture media. The quantity of the formazan dye, generated by dehydrogenases in cells, is directly proportional to the amount of cells. 

This kit is a colorimetric assay kit used to quantify cell proliferation and cytotoxicity.  It is a colorimetric assays used for the determination of cell viability in cell proliferation and cytotoxicity assays. The exceptionally water-soluble tetrazolium salt, WST-8, is decreased from dehydrogenase activities in cells to provide a yellow-color formazan dye, which can be soluble in the tissue culture media. 

The quantity of the formazan dye, generated by the actions of dehydrogenases in cells, is proportional to the number of cells present in the given sample. The detection sensitivity of CCK-8 is greater compared to other tetrazolium salts like MTT, XTT, MTS or even WST-1.    

 
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